DNA Sequencing from PCR Products
作者： Giorgio Basili , Serena Bonin , Isabella Dotti
DOI: 10.1007/978-3-642-17890-0_26
关键词:
摘要: This chapter provides a procedure and some advice for direct sequencing of PCR products from DNA extracted formalin fixed paraffin embedded (FFPE) tissues. A section in detail is dedicated to the analysis electropherograms. Generally, mutational requires extraction FFPE, amplification product purification sequencing. The particularly somatic mutations tumour tissues specifying importance analyzing limited amount non-mutated component order obtain good quality analyzable
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