Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae.
作者： Melanie L. Mayer , Steven P. Gygi , Ruedi Aebersold , Philip Hieter
DOI: 10.1016/S1097-2765(01)00254-4
关键词:
摘要: We have identified and characterized an alternative RFC complex RFC(Ctf18p, Ctf8p, Dcc1p) that is required for sister chromatid cohesion faithful chromosome transmission. Ctf18p, Dcc1p interact physically in a with Rfc2p, Rfc3p, Rfc4p, Rfc5p but not Rfc1p or Rad24p. Deletion of CTF18, CTF8, DCC1 singly combination (ctf18Deltactf8Deltadcc1Delta) leads to sensitivity microtubule depolymerizing drugs severe defect. Furthermore, temperature-sensitive mutations RFC4 result precocious separation. Our results highlight novel function the proteins support model which established at replication fork via polymerase switching mechanism replication-coupled remodeling chromatin.
elsevier.com
sci-hub.se 参考文章(59)
J.R. Lamb, W.A. Michaud, R.S. Sikorski, P.A. Hieter, Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. The EMBO Journal. ,vol. 13, pp. 4321- 4328 ,(1994) , 10.1002/J.1460-2075.1994.TB06752.X
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
Tomoyuki Tanaka, Jörg Fuchs, Josef Loidl, Kim Nasmyth, Cohesin ensures bipolar attachment of microtubules to sister centromeres and resists their precocious separation. Nature Cell Biology. ,vol. 2, pp. 492- 499 ,(2000) , 10.1038/35019529
Christoph Lengauer, Kenneth W. Kinzler, Bert Vogelstein, Genetic instabilities in human cancers Nature. ,vol. 396, pp. 643- 649 ,(1998) , 10.1038/25292
Frank Uhlmann, Friedrich Lottspeich, Kim Nasmyth, Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1 Nature. ,vol. 400, pp. 37- 42 ,(1999) , 10.1038/21831
S. L. Gerring, F. Spencer, P. Hieter, The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M. The EMBO Journal. ,vol. 9, pp. 4347- 4358 ,(1990) , 10.1002/J.1460-2075.1990.TB07884.X
P Hieter, C Connelly, S L Gerring, F Spencer, Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics. ,vol. 124, pp. 237- 249 ,(1990) , 10.1093/GENETICS/124.2.237
N Kouprina, E Kroll, V Larionov, A Kirillov, V Zakharyev, V Bannikov, CHL12, a gene essential for the fidelity of chromosome transmission in the yeast Saccharomyces cerevisiae. Genetics. ,vol. 138, pp. 1067- 1079 ,(1994) , 10.1093/GENETICS/138.4.1067
Vincent Guacci, Douglas Koshland, Alexander Strunnikov, A Direct Link between Sister Chromatid Cohesion and Chromosome Condensation Revealed through the Analysis of MCD1 in S. cerevisiae Cell. ,vol. 91, pp. 47- 57 ,(1997) , 10.1016/S0092-8674(01)80008-8
Mark S. Longtine, Amos Mckenzie III, Douglas J. Demarini, Nirav G. Shah, Achim Wach, Arndt Brachat, Peter Philippsen, John R. Pringle, Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae Yeast. ,vol. 14, pp. 953- 961 ,(1998) , 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U