Neuritogenic Effects of T Cell-Derived IL-3 on Mouse Splenic Sympathetic Neurons In Vivo

摘要: To determine the role played by lymphocytes and cytokines in growth of sympathetic neurons vivo, innervation cytokine levels were examined spleens SCID mice that lack T B cells. Splenic noradrenaline, nerve factor (NGF), IL-1β elevated mice. Immunohistochemical examination revealed density tyrosine hydroxylase-positive (TH+) fibers splenic central arteries was increased compared with wild-type C.B-17 mice, while had significantly fewer TH+ their periarteriolar lymphatic sheaths (PALS). Two weeks after injected cells, fiber staining PALS. IL-3 following cell reconstitution, administration anti-IL-3 Ab blocked above cell-induced increase Anti-IL-3 treatment also inhibited regeneration they chemically sympathetomized 6-hydroxydopamine. Depletion NK cells anti-asialo GM1 promoted there no significant changes between CD8+ cell-deficient β2-microglobulin knockout wild type. Our results suggest (probably CD4+ Th but not CTLs) play a regulating spleen; this effect appeared to be mediated, at least part, IL-3. On contrary, may exert an inhibitory on innervation.