Epigenetics and cortical spreading depression: changes of DNA methylation level at retrotransposon sequences

摘要: Cortical spreading depression (CSD) is an evolutionarily conserved phenomenon that involves a slow and self-propagating depolarization wave associated with spontaneous of electrical neuronal activity. CSD plays central role in the pathophysiology several brain diseases considered to be able promote "Preconditioning". This consists protecting itself against future injury by adaptation. Understanding molecular mechanisms underlying Preconditioning has significant clinical implications. We have already proposed long-lasting effects could related silencing retrotransposon sequences histone methylation. analyzed DNA methylation two sequences, LINE1 L1, their corresponding expression pattern after induction. Based on immunoprecipitation assay methylated (meDIP), we demonstrated hypermethylation both preconditioned rat cortex compared control 24 h Using quantitative PCR, also showed induction caused decrease transcript level sequences. Our data are consistent hypothesis epigenetic modifications Preconditioning-dependent neuroprotection increasing genome stability via