The effect of different dilution rates on post-thaw quality of ram semen frozen in two different eggyolk free extenders.
作者： E. Akçay , A. Daşki̇n , R. Kulaksız , Ç. Çebi̇ , K. Teki̇n
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摘要: Summary: The objective of the present study was to evaluate effects pre-freezing sperm concentration (200, 400 or 800x10 6 spermatozoa/ml) using two commercial extenders (Bioexcell ® and Andromed ) on post-thaw survival acrosomal status ram spermatozoa. Semen samples were obtained from 5 mature Karayaka rams (aged 2-3 yr) a total 30 ejaculates col-lected each male twice week for 3 weeks with aid an artificial vagina, during non-breeding season (February, win-ter). extended in Bioexcell diluents, loaded into 0.25 ml straws equilibrated at 4 o C 2 h. Straws frozen vapour liquid nitrogen then stored -196 C. After thawing (at 37 sec), motility, membrane integrity assessed. Pre-freezing influenced (P<0.001) frezability spermatozoa affected all vitro parameters 400x10 spermatozoa/ml negatively regardless extender. Decreas-ing 200x10
cabdirect.org参考文章(23)
ESE Hafez, None, Reproduction in farm animals ,(1974)
J. Nascimento, C.F. Raphael, A.F.C. Andrade, M.A. Alonso, E.C.C. Celeghini, R.P. Arruda, Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa Journal of Equine Veterinary Science. ,vol. 28, pp. 351- 358 ,(2008) , 10.1016/J.JEVS.2008.04.010
R Muiño, M Fernández, AI Peña, Post‐thaw Survival and Longevity of Bull Spermatozoa Frozen with an Egg Yolk‐based or Two Egg Yolk‐free Extenders after an Equilibration Period of 18 h Reproduction in Domestic Animals. ,vol. 42, pp. 305- 311 ,(2007) , 10.1111/J.1439-0531.2006.00784.X
S Salamon, W.M.C Maxwell, Storage of ram semen. Animal Reproduction Science. ,vol. 62, pp. 77- 111 ,(2000) , 10.1016/S0378-4320(00)00155-X
R. A. P. Harrison, H. M. Dott, G. C. Foster, Bovine serum albumin, sperm motility, and the “dilution effect” Journal of Experimental Zoology. ,vol. 222, pp. 81- 88 ,(1982) , 10.1002/JEZ.1402220111
S Bousseau, J.P Brillard, B Marquant-Le Guienne, B Guérin, A Camus, M Lechat, Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents. Theriogenology. ,vol. 50, pp. 699- 706 ,(1998) , 10.1016/S0093-691X(98)00175-7
J.C Samper, C.A Morris, Current methods for stallion semen cryopreservation: A survey Theriogenology. ,vol. 49, pp. 895- 903 ,(1998) , 10.1016/S0093-691X(98)00039-9
Yutaka FUKUI, Hirohide KOHNO, Tetsuro TOGARI, Mami HIWASA, Kentaro OKABE, Fertility after artificial insemination using a soybean-based semen extender in sheep. Journal of Reproduction and Development. ,vol. 54, pp. 286- 289 ,(2008) , 10.1262/JRD.20004
A.G. D'Alessandro, G. Martemucci, M.A. Colonna, A. Bellitti, POST-THAW SURVIVAL OF RAM SPERMATOZOA AND FERTILITY AFTER INSEMINATION AS AFFECTED BY PREFREEZING SPERM CONCENTRATION AND EXTENDER COMPOSITION Theriogenology. ,vol. 55, pp. 1159- 1170 ,(2001) , 10.1016/S0093-691X(01)00474-5
Ramanathan Kasimanickam, Kevin D. Pelzer, Vanmathy Kasimanickam, William S. Swecker, Craig D. Thatcher, Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs Theriogenology. ,vol. 65, pp. 1407- 1421 ,(2006) , 10.1016/J.THERIOGENOLOGY.2005.05.056