A quantitative procedure for the dissociation of adult mammalian muscle into mononucleated cells.
作者： Rose Yasin , Gisela Van Beers , Ditta Bulien , E.J. Thompson
DOI: 10.1016/0014-4827(76)90056-2
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摘要: Abstract A new technique for quantitative analysis of dissociated adult skeletal muscle is described. Using hamster biceps we obtained cell yields 1–4 × 10 6 viable cells/g muscle, 4–10% plating efficiency (% cells attached at 20 h in vitro) and spontaneously contracting myotubes day 7 which exhibited creatine phosphokinase specific activities 0.4 μmoles/min/ mg protein 25 °C. This can be applied to other tissue.
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