Genome-destabilizing and mutagenic effects of break-induced replication in Saccharomyces cerevisiae
作者： Angela Kay Deem
DOI:
关键词:
摘要: Deem, Angela Kay Ph.D., Purdue University, May 2011. Genome-destabilizing and Mutagenic Effects of Break-induced Replication in Saccharomyces cerevisiae. Major Professor: Cynthia V. Stauffacher. DNA suffers constant damage, leading to a variety lesions that require repair. One the most devastating is double-strand break (DSB), which results physical dissociation two pieces chromosome. Necessarily, cells have evolved number DSB repair mechanisms. mechanism break-induced replication (BIR), involves invasion one side broken chromosome into homologous template, followed by copying donor molecule through telomeric sequences. BIR an important cellular process implicated restart collapsed forks, as well various chromosomal instabilities. Furthermore, uniquely combines processive involving fork with This work employs system cerevisiae investigate genetic control, outcomes, frameshift mutagenesis associated initiated controlled HO-endonuclease Mutations POL32, encodes third, non-essential subunit polymerase (Pol ), RAD9 RAD24, participate damage checkpoint response, resulted defect characterized decreased increased
iupui.edu参考文章(232)
Karl Lenhard Rudolph, Melissa Millard, Marcus W. Bosenberg, Ronald A. DePinho, Telomere dysfunction and evolution of intestinal carcinoma in mice and humans. Nature Genetics. ,vol. 28, pp. 155- 159 ,(2001) , 10.1038/88871
Francesca Storici, L. Kevin Lewis, Michael A. Resnick, In vivo site-directed mutagenesis using oligonucleotides Nature Biotechnology. ,vol. 19, pp. 773- 776 ,(2001) , 10.1038/90837
Youri I. Pavlov, Polina V. Shcherbakova, Igor B. Rogozin, Roles of DNA polymerases in replication, repair, and recombination in eukaryotes. International Review of Cytology-a Survey of Cell Biology. ,vol. 255, pp. 41- 132 ,(2006) , 10.1016/S0074-7696(06)55002-8
Kenneth W. Kinzler, Lois L. Myeroff, James K. V. Willson, Sanford D. Markowitz, Ramon Parsons, Bo Liu, Bert Vogelstein, Microsatellite instability and mutations of the transforming growth factor β type II receptor gene in colorectal cancer Cancer Research. ,vol. 55, pp. 5548- 5550 ,(1995)
Akira Shinohara, Tomoko Ogawa, Stimulation by Rad52 of yeast Rad51- mediated recombination Nature. ,vol. 391, pp. 404- 407 ,(1998) , 10.1038/34943
T Formosa, B M Alberts, Purification and characterization of the T4 bacteriophage uvsX protein. Journal of Biological Chemistry. ,vol. 261, pp. 6107- 6118 ,(1986) , 10.1016/S0021-9258(17)38499-5
Martin L. Slater, Effect of Reversible Inhibition of Deoxyribonucleic Acid Synthesis on the Yeast Cell Cycle Journal of Bacteriology. ,vol. 113, pp. 263- 270 ,(1973) , 10.1128/JB.113.1.263-270.1973
Dring N. Crowell, David H. Huizinga, Angela K. Deem, Corey Trobaugh, Ryan Denton, Stephanie E. Sen, Arabidopsis thaliana plants possess a specific farnesylcysteine lyase that is involved in detoxification and recycling of farnesylcysteine The Plant Journal. ,vol. 50, pp. 839- 847 ,(2007) , 10.1111/J.1365-313X.2007.03091.X
Corrado Santocanale, John F. X. Diffley, A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication Nature. ,vol. 395, pp. 615- 618 ,(1998) , 10.1038/27001
Carol Wood Moore, Cleavage of Cellular and Extracellular Saccharomyces cerevisiae DNA by Bleomycin and Phleomycin Cancer Research. ,vol. 49, pp. 6935- 6940 ,(1989)