Preparation and characterization of anti-HIV nanodrug targeted to microfold cell of gut-associated lymphoid tissue

摘要: The human immunodeficiency virus 1 (HIV-1) still remains one of the leading life-threatening diseases in world. introduction highly active antiretroviral therapy has significantly reduced disease morbidity and mortality. However, most drugs have variable penetrance into viral reservoir sites, including gut-associated lymphoid tissue (GALT). Being largest organ, GALT plays a key role early HIV infection host-pathogen interaction. Many different treatment options been proposed to eradicate from GALT. it becomes difficult deliver traditional because its complex physiology. In this regard, we developed polymer-based Pluronic nanocarrier containing anti-HIV drug called efavirenz (EFV) targeting Microfold cells (M-cells) M-cells are specialized epithelial that predominantly present work, exploited paracellular transport property for targeted delivery tagged EFV, bioconjugated with anti-M-cell-specific antibodies (nanodrug). Preliminary characterization showed nanodrug (EFV-F12-COOH) is 140 nm size 0.3 polydispersion index, zeta potential particles was -19.38±2.2 mV. Further, dissolution study shown improved sustained release over free drugs. Binding M-cell also confirmed fluorescence microscopy vitro uptake studies. activity higher compared drug. This novel formulation able show EFV inhibit HIV-1 our vivo system by combining enteric-coated capsule technique via oral administration.