Molecular dissection of the role of histidine in nickel hyperaccumulation in Thlaspi goesingense (Halacsy)

作者: Michael W. Persans , Xiange Yan , Jean-Marc M.L. Patnoe , Ute Krämer , David E. Salt

DOI: 10.1104/PP.121.4.1117

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摘要: To understand the role of free histidine (His) in Ni hyperaccumulation Thlaspi goesingense, we investigated regulation His biosynthesis at both molecular and biochemical levels. Three T. goesingense cDNAs encoding following biosynthetic enzymes, ATP phosphoribosyltransferase (THG1, GenBank accession no. AF003347), imidazoleglycerol phosphate dehydratase (THB1, AF023140), histidinol dehydrogenase (THD1, AF023141) were isolated by functional complementation Escherichia coli auxotrophs. Northern analysis THG1, THD1, THB1 gene expression revealed that each is expressed roots shoots, but concentrations dosage times treatment used this study, these genes failed to show any Ni. We also unable observe increases concentration root, shoot, or xylem sap response exposure. X-ray absorption spectroscopy root shoot tissue from non-accumulator species arvense no major differences coordination tissues. therefore conclude phenotype not determined overproduction

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