Combined comparative and chemical proteomics on the mechanisms of levo-tetrahydropalmatine-induced antinociception in the formalin test.

摘要: This study investigated the mechanisms involved in antinociceptive action induced by levotetrahydropalmatine (L-THP) formalin test combined comparative and chemical proteomics. Rats were pretreated with L-THP oral route (40 mg/kg) 1 h before injection. The effect of was shown first second phases test. To address which inhibits formalin-induced nociception rats, proteomics applied. A novel high-throughput proteomic approach based on 2D-nano-LC-MS/MS applied to simultaneously evaluate deregulated proteins response treatment pain rats. Thousands identified, among 17 survived stringent filter criteria further included for functional discussion. Two (Neurabin-1 Calcium-dependent secretion activator 1) randomly selected, their expression levels confirmed Western Blots. results matched well those In present study, we also described development application immobilized beads bind targets. Following incubation cellular lysates, proteome interacting fixed identified. quite complementary. Although precise roles these identified moleculars L-THP-induced antinociception need indicated that associated signal transduction, vesicular trafficking neurotransmitter release, energy metabolism, ion transport play important