Comparison of myosin isoenzymes present in skeletal and cardiac muscles of the Arctic charr Salvelinus alpinus (L.). Sequential expression of different myosin heavy chains during development of the fast white skeletal muscle.

摘要: The expression of myosin isoforms and their subunit composition in the white skeletal body musculature Arctic charr (Salvelinus alpinus) different ages (from 77-day embryos until about 5 years old) was studied at protein level by means electrophoretic techniques. Myosin from muscle displayed three types light chain during all developmental stages examined: two chains type 1 (LClF) differing both apparent molecular mass PI, one 2 (LC2F) 3 (LC3F). fastest-migrating form LClF seemed to be predominant embryonic eleutheroembryonic periods. slowest-migrating LC1 F 5-year-old fish. Between year 4 years, LC1F were present similar amounts. Cardiac as well red 3-year-old fish had chain. atria ventriculi indistinguishable two-dimensional electrophoresis, but muscle. Neither cardiac nor coexpressed with any examined. Two heavy bands resolved SDS/glycerol/polyacrylamide gel electrophoresis extract embryos. One minor other, most abundant, band comigrated only found extracts older One-dimensional Staphylococcus aureus V8 protease peptide mapping these revealed some differences development tentatively interpreted follows. amounts may represent an early that is replaced a late or foetal eleutheroembryos. appears resorption yolk sack beginning free-swimming stage. A new chain, termed neonatal probably expressed around hatching, age. another type, named 11-1 whose fragments persisted rest period under study. called 11-2, apparently onwards. further transition observed between attributed appearance 11-3. New peptides appeared V8-protease-treated charr. These are considered due yet 11-4. In conclusion, minimum six sequentially native adults differed subunits, closely related because small map patterns. adult seven displaying five patterns: white, red, pink, atrial ventricular chains. molecule made up four Differences give rise [l]. During muscles, ex