Encapsulation in a sol-gel matrix of lipase from Aspergillus niger obtained by bioconversion of a novel agricultural residue.

摘要: Lipase from Aspergillus niger was obtained the solid-state fermentation of a novel agroindustrial residue, pumpkin seed flour. The partially purified enzyme encapsulated in sol–gel matrix, resulting an immobilization yield 71.4 %. optimum pH levels free and enzymes were 4.0 3.0, respectively. showed greater thermal stability at temperatures 45 60 °C than enzyme. positive influence encapsulation process observed on enzyme, since longer half-life t 1/2 lower deactivation constant with lipase when compared lipase. Kinetic parameters found to follow Michaelis–Menten equation. K m values indicated that reduced enzyme–substrate affinity V max about 31.3 % operational investigated, showing 50 % relative activity up six cycles reuse 3.0 37 °C. Nevertheless, production residue associated efficient method, which promotes good catalytic properties makes economically viable for future industrial applications.