DNA curvature and deformation in protein–DNA complexes: A step in the right direction

摘要: The sequence target of E2 has the general form ACCGNNNNCGGT, where N4 is variable and does not contact protein. Binding protein yields bends toward major grooves at dyad-symmetric ACCG/CGGT elements (4, 5). result primarily from roll, which measures rotation a base pair plane about its long axis. This motion creates an angle, narrowing groove for positive between two otherwise parallel adjacent pairs. Because centers sequences are less than full helical turn apart, writhed curve rather planar bend DNA axis results.