DNase-chip: A high-resolution method to identify DNase I hypersensitive sites using tiled microarrays

摘要: Mapping DNase I hypersensitive sites is an accurate method of identifying the location gene regulatory elements, including promoters, enhancers, silencers and locus control regions. Although Southern blots are traditional sites, conventional manual not readily scalable to studying large chromosomal regions, much less entire genome. Here we describe DNase-chip, approach that can rapidly identify for any region interest, or potentially genome, by using tiled microarrays. We used DNase-chip accurately from a representative 1% human genome in both primary immortalized cell types. found although most were present types studied, some them cell-type specific. This be applied globally targeted fashion tissue species with sequenced