Nanospheres based on PLGA/amphiphilic cyclodextrin assemblies as potential enhancers of Methylene Blue neuroprotective effect

摘要: Methylene Blue (MB) has recently showed beneficial effects towards neurological disorders such as Alzheimer’s and Parkinson’s diseases. Intravenous administration of MB could be difficult because its poor cooperation with patients, thus entrapment in a carrier system improve compliance. PLGA nanospheres have been proposed delivery for MB, but they suffer from low encapsulation efficiency rapid release their cargo. Here, we design high affinity hydrophilic based on non-ionic amphiphilic cyclodextrin (SC6OH) an additional component. Interaction between SC6OH was firstly investigated by UV-vis spectroscopy steady-state emission fluorescence aqueous solution. PLGA/SC6OH loaded were prepared nanoprecipitation/solvent displacement method characterized STEM FTIR-ATR analysis. They display sizes about 200 nm, higher respect to without SC6OH. This latter modulates the profiles nanospheres, producing sustained five days. In vitro biological studies human neuroblastoma SH-SY5Y cells demonstrated that did not affect cell viability. addition, loaded-PLGA/SC6OH produced significant neuroprotection against metabolic iodoacetic acid, especially presence NADH electron donor.