Development of serum-free media for lepidopteran insect cell lines

摘要: Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part one most successful eukaryotic expression systems and is increasingly used industrially on a large scale. Therefore, there constant need for convenient low-cost media capable supporting good growth ensuring high yield baculovirus as well strong recombinant proteins. Vertebrate sera or invertebrate hemolymph were supplements in first-generation media. These supplements, however, are cumbersome expensive routine large-scale culture; thus, their use now circumvented by substituting factors present these with serum-free substances. Such non-serum typically non-animal origin include protein hydrolysates, lipid emulsions, specialized substances (e.g., surfactants shear damage protecting chemicals). complement defined, synthetic basal medium ensure that fundamental nutritional needs cells satisfied. Although significant number proprietary low-protein protein-free market, lack information concerning detailed composition drawback adoption different applications, including adaptation metabolic kinetic analysis monitoring given based bioprocess. Hence, wide appeal formulating rational assessment requirements lepidopteran during both production phases. Techniques such statistical experimental design genetic algorithms adapted cellular behavior bioreactor operation mode (batch, fed-batch, perfusion) permit formulation versatile serum- techniques illustrated recent developments cultivation commercially important Spodoptera frugiperda Trichoplusia ni lines.