Glomerular expression of transforming growth factor–β (TGF-β) isoforms in mice lacking CD2-associated protein
作者： Robert P. Woroniecki , Mario Schiffer , Andrey S. Shaw , Frederick J. Kaskel , Erwin P. Bottinger
DOI: 10.1007/S00467-005-2102-7
关键词:
摘要: Mice lacking CD2-associated protein (CD2AP−/−) develop glomerular lesions resembling human focal segmental glomerulosclerosis (FSGS) between 3–4 weeks of age and die approximately 2 weeks later from massive proteinuria renal failure. The mechanisms involved in the injury this model are unclear. In study, we used laser capture microdissection (LCM) real-time PCR, examined expression TGF-s isoforms CD2AP−/− mice at level isolated glomeruli. Total RNA yield cryosections 30 glomeruli was 10.71 ng (SD, 5.45) CD2AP+/+ group (n =7), 4.20 ng 2.04) =8), p =0.008. Expression TGF-s1 mRNA increased 1.5-fold whole kidney (p =0.030), twofold =0.026). Whole receptor I (RI) II (RII) not different animals, but it samples by 4.38-fold =0.001) 11.37-fold =0.0163), respectively. By using LCM confirmed levels previously described our sieving CD2AP KO underscored importance local factors development glomerulosclerosis.
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sci-hub.se 参考文章(26)
Valentina M. Factor, Erwin P. Böttinger, Snorri S. Thorgeirsson, Jeffrey B. Kopp, Nancy Sanderson, Paul E. Klotman, Peter Nagy, Miklos Mozes, Transgenic mice with increased plasma levels of TGF-beta 1 develop progressive renal disease. Laboratory Investigation. ,vol. 74, pp. 991- 1003 ,(1996)
R. F. Bonner, CELL SAMPLING: Laser Capture Microdissection: Molecular Analysis of Tissue Science. ,vol. 278, pp. 1481- 1483 ,(1997) , 10.1126/SCIENCE.278.5342.1481
Rachel A. Craven, Nick Totty, Patricia Harnden, Peter J. Selby, Rosamonde E. Banks, Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: evaluation of tissue preparation and sample limitations. American Journal of Pathology. ,vol. 160, pp. 815- 822 ,(2002) , 10.1016/S0002-9440(10)64904-8
Wayne A. Border, Seiya Okuda, Lucia R. Languino, Michael B. Sporn, Erkki Ruoslahti, Suppression of experimental glomerulonephritis by antiserum against transforming growth factor β1 Nature. ,vol. 346, pp. 371- 374 ,(1990) , 10.1038/346371A0
Mario Schiffer, Peter Mundel, Andrey S. Shaw, Erwin P. Böttinger, A Novel Role for the Adaptor Molecule CD2-associated Protein in Transforming Growth Factor-β-induced Apoptosis * Journal of Biological Chemistry. ,vol. 279, pp. 37004- 37012 ,(2004) , 10.1074/JBC.M403534200
Michael L Dustin, Michael W Olszowy, Amy D Holdorf, Jun Li, Shannon Bromley, Naishadh Desai, Patricia Widder, Frederick Rosenberger, P.Anton van der Merwe, Paul M Allen, Andrey S Shaw, A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts. Cell. ,vol. 94, pp. 667- 677 ,(1998) , 10.1016/S0092-8674(00)81608-6
Jeong M Kim, Hui Wu, Gopa Green, Cheryl A Winkler, Jeffrey B Kopp, Jeffrey H Miner, Emil R Unanue, Andrey S Shaw, CD2-associated protein haploinsufficiency is linked to glomerular disease susceptibility. Science. ,vol. 300, pp. 1298- 1300 ,(2003) , 10.1126/SCIENCE.1081068
Yeong Hoon Kim, Meera Goyal, David Kurnit, Bryan Wharram, Jocelyn Wiggins, Lawrence Holzman, David Kershaw, Roger Wiggins, Podocyte depletion and glomerulosclerosis have a direct relationship in the PAN-treated rat Kidney International. ,vol. 60, pp. 957- 968 ,(2001) , 10.1046/J.1523-1755.2001.060003957.X
Neng-Yao Shih, Jun Li, Vladimir Karpitskii, Ancho Nguyen, Michael L Dustin, Osami Kanagawa, Jeffrey H Miner, Andrey S Shaw, Congenital Nephrotic Syndrome in Mice Lacking CD2-Associated Protein Science. ,vol. 286, pp. 312- 315 ,(1999) , 10.1126/SCIENCE.286.5438.312
Michael B. Sporn, Anita B. Roberts, Peptide growth factors are multifunctional Nature. ,vol. 332, pp. 217- 219 ,(1988) , 10.1038/332217A0