作者: Robert P. Woroniecki , Erwin P. Bottinger
DOI: 10.1007/978-1-59745-352-3_6
关键词:
摘要: Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis disease glomeruli and tubules. This technique readily applicable to study mRNA from isolated tubules human biopsy material. In this chapter we present a "cook-book" practical approach utilizing LCM combination with isolation downstream applications nephrology.