Laser-capture microdissection of renal tubule cells and linear amplification of RNA for microarray profiling and real-time PCR.
作者： Susie-Jane Noppert , Susanne Eder , Michael Rudnicki
DOI: 10.1007/978-1-61779-163-5_21
关键词:
摘要: Abstract Laser-capture microdissection and transcriptional profiling have enabled compartment- cell-specific analysis of gene expression in chronic kidney disease, thus facilitating the investigation pathophysiological associations between glomerular, tubular, interstitial structures. Due to pico- nanogram amounts RNA isolated from LCM-captured material linear amplification protocols are necessary prior real-time PCR microarray analysis. In this chapter, we describe isolation renal tubule cells cryocut sections routine biopsies, for downstream purposes.
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