Analysis of segmental renal gene expression by laser capture microdissection

摘要: Analysis of segmental renal gene expression by laser capture microdissection. Background The study normal physiology has been greatly aided microdissection techniques that have delineated the exceptional functional and cellular heterogeneity both along nephron between different populations. These are not widely used to injury as is difficult because tissue necrosis or fibrosis. We developed a procedure detect specific in locations kidney histologic sections. Methods anatomic specificity (LCM) was employed with sensitivity reverse transcriptase-polymerase chain reaction (RT-PCR). Results LCM/RT-PCR detected mRNA for podoplanin 2% single glomerulus, rat basic amino acid transporter 6% cross-section proximal straight tubule, renin eight convoluted tubule cross-sections. could isolate pure populations tubules, thick ascending limbs from sections, although collecting ducts be isolated. localized ischemia-reperfusion–induced induction KC/interleukin-8 primarily medullary limb, transforming growth factor-!; (TGF-!;) glomeruli patient membranous glomerulonephropathy. Conclusions When an appropriate spot size, can measure parts alterations steady-state levels animal models disease. applications, limitations, refinements this approach discussed.