Laser Capture Microdissection
作者： Michael R. Emmert-Buck , Robert F. Bonner , Paul D. Smith , Rodrigo F. Chuaqui , Zhengping Zhuang
DOI: 10.1126/SCIENCE.274.5289.998
关键词:
摘要: Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section complex, heterogeneous tissue. In this technique, transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface tissue on standard glass histopathology slide; carbon dioxide laser pulse then specifically activates above cells interest. Strong focal adhesion allows selective targeted cells. Multiple examples LCM transfer and analysis, including polymerase chain reaction amplification DNA RNA, enzyme recovery transferred are demonstrated.
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