Allelic Loss on Chromosome 8p12–21 in Microdissected Prostatic Intraepithelial Neoplasia
作者： Cathy D. Vocke , Michael R. Emmert-Buck , Lance A. Liotta , W. Marston Linehan , Paul H. Duray
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摘要: The development and progression of human prostate cancer is associated with genetic abnormalities in tumor cells. Inactivation suppressor genes due to allelic loss thought be an important mechanism gene alteration prostatic neoplasms. In this study we examined on chromosome 8p12-21 microdissected samples normal epithelium, high grade intraepithelial neoplasia (PIN), invasive carcinoma from the same patients. Tissue microdissection under direct microscopic visualization procures pure populations cells interest, including small lesions such as PIN. Among 30 patients concomitant PIN, found heterozygosity 63% (34 54) foci PIN 90.6% (29 32) tumors, suggesting that may early stages development. Several cases which multiple patient were sampled showed different patterns loss. Fifty-five % (16 29) carcinomas contained a potential precursor focus based pattern. Our results are consistent hypothesis arises multifocally within gland, subset these progress become carcinoma.
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