Functional maturation of recent thymic emigrants in the periphery: development of alloreactivity correlates with the cyclic expression of CD45RC isoforms.
作者： Chun-Ping Yang , Eric B. Bell
关键词:
摘要: The transition from fully developed CD4+CD8- single-positive (SP) thymocytes into mature recirculating peripheral T cells is both poorly understood with regard to the expression of restricted isoforms (CD45R) leukocyte common antigen and in terms cell function. present investigation monitored extrathymic development SP euthymic recipients using allotype-marked donor monoclonal antibody OX22 which recognizes an epitope on C exon rat CD45R. We established that donor-derived blood 1 day later bore phenotype injected (CD4+ Thy-1+ CD45RC-). identical were also thoracic duct lymph uninjected rats, suggesting CD45RC- represent recent thymic emigrants (RTE) have migrated periphery their own accord. During maturation RTE lost Thy-1 within 3 days expressed CD45RC+ high molecular weight isoform by 7; between 8 14 a proportion (25%-30%) once again (CD45RC-). (CD45RC-) CD4 via intermediate was accompanied at each stage increased ability maturing induce skin allograft rejection. Unexpectedly, subsequent loss isoform, following presumed encounter, associated significant reduction this Thy-1-CD45RC- subpopulation effect graft cyclic CD45RC immature fact low found (unquestionably naive) antigen-experienced cells, makes it unlikely uniquely identifies memory least rat.
sci-hub.se 参考文章(49)
M T Drayson, S M Sparshott, E B Bell, S V Hunt, The origin of T cells in permanently reconstituted old athymic nude rats. Analysis using chromosome or allotype markers. Immunology. ,vol. 68, pp. 547- 556 ,(1989)
F. Powrie, D. Mason, Subsets of Rat CD4+ T Cells Defined by Their Differential Expression of Variants of the CD45 Antigen: Developmental Relationships and In Vitro and In Vivo Functions Immunological Memory. ,vol. 159, pp. 79- 96 ,(1990) , 10.1007/978-3-642-75244-5_5
M. H. Brown, L. A. Terry, P. C. L. Beverley, The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45. Immunology. ,vol. 64, pp. 331- 336 ,(1988)
R. E. Callard, M. H. Brown, S. H. Smith, D. Rowe, P. C. L. Beverley, Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. ,vol. 58, pp. 63- 70 ,(1986)
A M Martin, N Yamashita, L T Clement, The functionally distinct subpopulations of human CD4+ helper/inducer T lymphocytes defined by anti-CD45R antibodies derive sequentially from a differentiation pathway that is regulated by activation-dependent post-thymic differentiation. Journal of Immunology. ,vol. 141, pp. 1464- 1470 ,(1988)
Ellen S. Vitetta, William T. Lee, Xiao Ming Yin, Functional and ontogenetic analysis of murine CD45Rhi and CD45Rlo CD4+ T cells. Journal of Immunology. ,vol. 144, pp. 3288- 3295 ,(1990)
T A Springer, M W Makgoba, D Stephany, S O Sharrow, S Shaw, M E Sanders, H A Young, Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN-gamma production. Journal of Immunology. ,vol. 140, pp. 1401- 1407 ,(1988)
W F Chen, R Scollay, K Shortman, The functional capabilities of cells leaving the thymus. Journal of Immunology. ,vol. 132, pp. 25- 30 ,(1984)
L M Pilarski, J F Krowka, H M Serra, J A Ledbetter, Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event. Journal of Immunology. ,vol. 140, pp. 1435- 1441 ,(1988)
Peter Beverley, Immunological memory in T cells. Current Opinion in Immunology. ,vol. 3, pp. 355- 360 ,(1991) , 10.1016/0952-7915(91)90038-3