Hydrogen-rich saline attenuates chemotherapy-induced ovarian injury via regulation of oxidative stress

摘要: Hydrogen has been reported to exert a therapeutic effect in several diseases due its antioxidative, anti-inflammatory and anti-apoptotic properties. The aim of the present study was investigate whether hydrogen-rich saline treatment could attenuate ovarian damage induced by cisplatin. A total 240 adult, virgin, female Sprague Dawley rats, weighing 180-220 g, were randomly divided into four groups (n=60 per group): Control (Con), control + (Con H2), cisplatin-induced injury (OI) (OI H2). Cisplatin diluted immediately before use. In OI H2 groups, rats administered dose cisplatin on 1st 7th days. Con intraperitoneally injected with (10ml/kg body weight) once day over 2-week period. On 14th, 28th 42nd days (T1, T2 T3) after injection, femoral vein blood collected. At end experiment, homogenates prepared, samples used for estrogen (E2), follicle-stimulating hormone (FSH), superoxide dismutase (SOD), catalase (CAT) malondialdehyde (MDA) examination. addition, (n=10 group) sacrificed bilateral ovary removal; one fixed formalin follicle-counting analysis, while other nuclear factor erythroid 2-related 2 (Nrf2) detection western blotting. Hydrogen-rich attenuated FSH release, elevated level E2, improved development follicles, reduced cortex at T1, T3 rats. oxidative stress increasing levels oxidation products attenuating activity antioxidant enzyme, which be reversed treatment. Furthermore, regulated Nrf2 protein expression damage. conclusion, exerts protective against reducing MDA SOD CAT activity. Ovarian chemotherapy involves activation Nrf2.