Mechanism of enzymic hydrolysis I. Role of the acidic group in the estetratic site of acetylcholinesterase

摘要: Measurements of the inhibition acetylcholinesterase by prostigmine as a function pH under non-competitive conditions reveal that basic group in esteratic site enzyme is essential for formation substrate complex but tha] acid not significantly involved. The hydrolytic process which follows enzyme-substrate complex. These conclusions are accordance with previously developed mechanism catalyzed hydrolysis. The measured dissociation constant and free enzyme.