Functional Vasoactive Intestinal Polypeptide (VIP) Receptors in Human Neuroblastoma Subclones That Contain VIP Precursor mRNA and Release VIP-like Substances
作者： JM Muller , SJ Lolait , VC Yu , W Sadee , JA Waschek
DOI: 10.1016/S0021-9258(19)84897-4
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摘要: Abstract Three phenotypically distinct subclones (SH-SY-5Y, SH-EP, SH-IN) of the human neuroblastoma cell line SK-N-SH were found to possess vasoactive intestinal polypeptide (VIP) precursor mRNA, release immunoreactive VIP, and express high-affinity VIP receptors coupled adenylate cyclase. The apparent molecular mass for receptor polypeptide, as determined by covalent cross-linking 125I-VIP, was 49 kDa. After 2 days in culture, a concentration equivalent binding affinity its medium two these clones (SH-IN SH-EP). Conditioned from SH-IN cells competitively displaced 125I-VIP increased cAMP levels SH-EP cells, indicating that all necessary components potential autocrine action exist cells. numerous passages, subclone converted phenotype which mRNA immunoreactivity no longer detectable. In correlation, number increased, EC50 stimulation production shifted lower concentration. This points possibility continuous presence endogenous earlier passage causes modification responsiveness VIP.
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