Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA.

摘要: The adenovirus 5 mutant H5dl337 lacks 146 base pairs within early region 1B. deletion removes a portion of the encoding E1B 21,000-molecular-weight (21K) polypeptide, but does not disturb E1B-55K/17K coding region. virus is slightly defective for growth in cultured HeLa cells, which its final yield reduced ca. 10-fold compared with wild-type virus. displays striking phenotype cells. onset cytopathic effect dramatically accelerated, and both host cell viral DNAs are extensively degraded late after infection. This defect has been described previously variety mutants termed cytocidal (cyt) phenotype. serves to map this loss E1B-21K polypeptide function. In addition productive cycle, unable transform rat cells at normal efficiency.