Systems metabolic engineering design: Fatty acid production as an emerging case study

摘要: Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids chain lengths from C6 C16 are propitious intermediates the catalytic synthesis industrial diesel-like biofuels. The abundance genetic information available Escherichia coli specifically, fatty acid metabolism in E. coli, supports this bacterium as a promising host engineering biocatalyst acids. Recent successes rooted different features systems metabolic strain design high-yielding medium producing strains provide an emerging case study methods effective design. Classical synthetic biology approaches enabled distinct paths towards strain. Here we highlight rational process biology, integrated computational experimental approach carboxylic production, alternative method. Additional challenges inherent achieving optimal commercialization chain-length will likely require collection strategies engineering. Not only continued advancement result these highly productive more quickly, knowledge extend rapidly platform with alternate chain-lengths functionalities.