Pancreatic effects of ethionine: blockade of exocytosis and appearance of crinophagy and autophagy precede cellular necrosis

摘要: Young female mice fed a choline-deficient, ethionine-supplemented (CDE) diet for 24 h develop hemorrhagic pancreatic necrosis with 5-day mortality rate of approximately 50%. At the end administration, in vivo discharge digestive enzymes is blocked, images exocytosis and luminal membrane recycling disappear, zymogen granules accumulate within acinar cells. The general ultrastructure, however, remains well preserved, protein synthesis normal, intracellular transport secretory proteins only slightly retarded. Thus, CDE does not affect phenomenon fusion-fission but specifically inhibits that associated exocytosis. Twenty-four hours after withdrawal diet, into lysosomes (crinophagy) can be observed, and, latr, autophagocytosis noted. Finally, shortly before onset necrosis, cells nearly normal appearance are noted to scattered among showing varying degrees lesion up complete disruption. CDE-induced results from sequence events: blockade evolves crinophagy autophagy, which might lead lysosomal activation zymogens.