On-chip real-time detection and quantification of reactive oxygen species in MCF-7 cells through an in-house built fluorescence microscope

摘要: Abstract Reactive oxygen species (ROS) are formed by several different reactions and play a major role in cell cycling as messenger normal transduction have diverse biological effects when high concentration. ROS may trigger DNA damage, mutation, inflammation, proliferation, ultimately they induce carcinogenesis. This study aims to design, fabricate, develop novel on-chip detection based on mounted in-house built fluorescence microscope microfluidics, which has not been reported previously. An 3D printed digital portable type for developed. The combined use of the glass-based microfluidic chip both enabled provide high-quality images living cells with ease controlling handling sample. As an evidence microscope, breast cancer were loaded 2′,7′-dichlorofluorescein diacetate (DCFDA, 50 μM) 45 min produces green upon reaction intracellular ROS. Fluorescence MCF-7 captured obtain profile generation 1 day. LabVIEW 2019 was used along its high-performance image analysis IMAQ Vision tools. viability performed LIVE/DEAD assay Luna counter. developed platform provides real-time monitoring quantification A strong linear correlation between concentrations versus recorded. result recommends that current in-vitro assessment cycle progression evaluation variety drugs.