Polyphasic characterization of bacterial community in fresh cut salads

摘要: In the present work we describe a polyphasic study of bacterial community in fresh cut salads packaged under ordinary and modified atmospheres. Samples were aseptically collected at 0, 3, 6 days storage analysed both by culture-dependent -independent methods. DNA extracted from salad samples was used as template for PCR amplification 16 S rRNA gene; products analyzed denaturing gradient gel electrophoresis (DGGE); finally, clone libraries gene constructed. Results plating count revealed significant increase all microbial loads fresh-cut OA that growth different groups significantly affected conditions applied MA packaging. A constant presence, throughout storage, pathogenic bacteria highlighted PCR-DGGE analysis. Therefore, approach allowed us to characterize main species involved better understand their dynamics storage.