Levels of antibodies to Plasmodium falciparum sporozoite surface antigens reflect malaria transmission rates and are persistent in the absence of reinfection.

摘要: Antibodies reacting with Plasmodium falciparum sporozoite surface antigens were measured by an immunofluorescence assay using wet preparations of sporozoites attached to poly-L-lysine-treated glass slides, a procedure which was found be more specific than one glutaraldehyde-treated and dried preparations. Subjects recovering from first attack negative. In two African villages differed in the level at mosquitoes transmit disease (1 100 infective bites per year individual), both prevalence age group levels anti-sporozoite antibodies markedly, as follows. low-transmission area, these not detected subjects aged 2 10 years; thereafter, increased gradually subject reached 90% 50 80 years. high-transmission all studied, including younger ones, positive. Anti-sporozoite antibody independent directed against blood stages. On average, mean titers equal 1/16 village 1/1,650 second one. These results suggest that stage-specific reflect cumulative number inoculated humans may therefore have useful epidemiological applications. addition, presence sera adults collected different times after departure endemic area indicates they last for several During course this study, we observed heterogeneity labeling parasite populations prepared mosquito salivary glands. This raises question possible qualitative or quantitative antigenic differences between other.