Cytology and autoradiography of estrogen-induced differentiation of avian endosteal cells

摘要: The endosteal reaction, the initial step in formation of medullary bone, was investigated femurs estrogen-treated male Japanese quail. Morphologically, cells were an undifferentiated state until 30 h after estrogen treatment and showed characteristics resembling those resting cells. Many preosteoblasts seen on endosteum at 33 h, whereas mitotic figures fully differentiated osteoblasts recognized 36 estrogen. observed among endosteum. Autoradiographs that number labeled by [3H]thymidine injected 1 before sacrifice maximal 27 administration decreased markedly h. When a single injection given 26 estrogen, highest percent later (27 estrogen). Labeled 7 (33 estrogen) 10 (36 estrogen), respectively. Our results show under influence are induced to maximally synthesize DNA about These appear modulate into 6 then divide via mitosis become within additional 3 development bone occurs sequential predictable manner, which makes it useful system for studying basic problems cell differentiation.