Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation.

摘要: Human periodontal ligament stem cells (hPDLSCs) are ideal seed for regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to improvement regeneration tissue engineering. Tandem Mass Tag quantitative proteomics was utilized reveal temporal expression pattern hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) were clustered functional annotated by Gene Ontology (GO) terms. Pathway enrichment analysis performed based Kyoto Encyclopedia Genes Genomes database, followed predicted activation using Ingenuity Analysis software. Interaction networks redox-sensitive signalling pathways oxidative phosphorylation (OXPHOS) conducted hub SOD2 validated with western blotting. total 1024 DEPs identified in 5 distinctive clusters representing tendencies. The GO results indicated that different tendencies show functions. found OXPHOS significantly involved, which further continuous activation. Redox-sensitive status showed associations various degrees, especially sirtuin pathway. SOD2, an important component pathway, displays a persistent increase osteogenesis. Data available via ProteomeXchange identifier PXD020908. This is first in-depth proteomic hPDLSCs. It demonstrated regulatory mechanism hPDLSC osteogenesis might provide new perspective research