Human papillomavirus and p53 mutational status as prognostic factors in head and neck carcinoma

摘要: Background Mutations of the p53 tumor-suppressor gene are common in squamous cell carcinoma head and neck (SCCHN) may portend a worse prognosis. Human papillomavirus (HPV) represents another potential prognostic factor for SCCHN. The oncogenic HPV be due to ability its E6 oncoprotein promote degradation wild-type protein. We wish determine whether there is lower incidence mutations HPV-positive versus HPV-negative tumors, if and/or status has an impact on survival. Methods Thirty-two SCCHN specimens were analyzed using single-strand conformational polymorphism (SSCP) analysis followed by DNA sequencing. all was evaluated use polymerase chain reaction with consensus primers Southern blot hybridization. Pertinent clinical information obtained from chart review. Results Nonsilent present 2 15 (13%) tumors compared 6 17 (35%) (p = .229; Fisher's exact test, odds ratio .28). A survival advantage found between .0264) wild type mutant .01) univariate log rank analysis. When stratified according both status, statistically significant difference observed largely because 100% HPV-positive/p53 group .003). Conclusions This preliminary study supports notion that presence confers among HNSCC patients, particularly when type. © 2002 Wiley Periodicals, Inc. Head Neck 24: 841–849,