Collagen type I synthesized by pancreatic periacinar stellate cells (PSC) co-localizes with lipid peroxidation-derived aldehydes in chronic alcoholic pancreatitis.

摘要: Chronic alcoholic pancreatitis (CAP) is characterized by progressive pancreatic fibrosis and loss of the acinar cell mass, but pathogenesis in human poorly understood. It has been recently suggested that lipid peroxidation-derived aldehydes such as 4-hydroxynonenal (HNE) are involved tissue damage other organs. The aim this study was to evaluate role oxidative stress development alcohol-induced humans, assess contribution periacinar stellate cells (PSC) vivo synthesis extracellular matrix components during CAP. Lipid peroxidation evaluated specimens obtained from patients with CAP who underwent surgical procedures, immunohistochemistry using a monoclonal antibody directed against HNE–protein adducts. Immunohistochemical determination collagen type I, α-smooth muscle actin (α-SMA), β subunit platelet-derived growth factor (PDGF-Rβ) also performed. In addition, mRNA expression procollagen PDGF-Rβ, transforming factor-β1 (TGF-β1) situ hybridization. CAP, increased formation adducts evident adjacent interlobular connective stained positively for I. HNE staining absent normal pancreas. Several non-parenchymal underlay HNE-stained cells. Those PSC α-SMA PDGF-Rβ showed active I specific mRNAs. pattern reflected observed immunostaining, showing amounts transcripts PSC. TGF-β1 mRNA were found several types including PSC, acinar, ductal These results indicate significant phenomena occur they associated Copyright © 2000 John Wiley & Sons, Ltd.