Development of new technologies directed toward superfine gene analysis

Mitsuo Sekine , Akihiro Ohkubo , Akio Kobori , Kohji Seio
Tanpakushitsu kakusan koso. Protein nucleic acid enzyme 49 2978

2004
Synthesis of 2'-O-alkylcarbamoylethyl-modified oligonucleotides with enhanced nuclease resistance that form isostable duplexes with complementary RNA.

Kohji Seio , Takahito Tomori , Tomohiro Kishimura , Yoshiaki Masaki
Bioorganic & Medicinal Chemistry Letters 35 127779

2021
New thermolytic carbamoyl groups for the protection of nucleobases.

Akihiro Ohkubo , Rintaro Kasuya , Kenichi Miyata , Hirosuke Tsunoda
Organic and Biomolecular Chemistry 7 ( 4) 687 -694

14
2009
Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions

Hirosuke Tsunoda , Tomomi Kudo , Yoshiaki Masaki , Akihiro Ohkubo
Nucleic Acids Research 39 ( 7) 2995 -3004

5
2011
A new silyl Ether-type linker useful for the automated synthesis of oligonucleotides having base-labile protective groups

Akio Kobori , Kenichi Miyata , Masatoshi Ushioda , Kohji Seio
Chemistry Letters 31 ( 1) 16 -17

12
2002
2005
Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine.

Kohji Seio , Takashi Kanamori , Munefumi Tokugawa , Hiroki Ohzeki
Bioorganic & Medicinal Chemistry 21 ( 11) 3197 -3201

6
2013
O-selective condensation using P-N bond cleavage in RNA synthesis without base protection.

Akihiro Ohkubo , Yasukazu Kuwayama , Tomomi Kudo , Hirosuke Tsunoda
Organic Letters 10 ( 13) 2793 -2796

10
2008
Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine.

Ken-ichi Miyata , Ryuji Tamamushi , Hirosuke Tsunoda , Akihiro Ohkubo
Organic Letters 11 ( 3) 605 -608

11
2009
Mild and Facile Deprotection for the Synthesis of Oligodeoxynucleotide Incorporating a 6-O-Ethyl-deoxyguanosine

Kohji Seio , Tomoe Negishi , Kazuo Negishi , Mitsuo Sekine
Letters in Organic Chemistry 2 ( 2) 179 -183

3
2005
2004
Synthesis and Stability of 1-Phenylethenyl Phosphate Derivatives and their Phosphoryl Transfer Activity

Tomohisa Moriguchi , Kazuhisa Okada , Kohji Seio , Mitsuo Sekine
Letters in Organic Chemistry 1 ( 2) 140 -144

7
2004
Synthesis of Enol Adenosine 5-Phosphate Derivatives by the Perkow Reaction of a Silylated Adenosine 5-Phosphonate Derivative with α-Halo Ketones

Mitsuo Sekine , Tomohisa Moriguchi , Kazuhisa Okada , Kohji Seio
Letters in Organic Chemistry 1 ( 3) 246 -248

2004
Chemical Synthesis of a 5‘-Terminal TMG-Capped Triribonucleotide m32,2,7G5‘pppAmpUmpA of U1 RNA

Mitsuo Sekine , Michinori Kadokura , Takahiko Satoh , Kohji Seio
Journal of Organic Chemistry 61 ( 13) 4412 -4422

21
1996
Chemical Synthesis and Conformational Properties of a New Cyclouridylic Acid Having an Ethylene Bridge between the Uracil 5-Position and 5‘-Phosphate Group

Kohji Seio , Takeshi Wada , Kensaku Sakamoto , Shigeyuki Yokoyama
Journal of Organic Chemistry 61 ( 4) 1500 -1504

13
1996
Synthesis of 2'-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides.

Yoshiaki Masaki , Keishi Yamamoto , Takeshi Inde , Keita Yoshida
Bioorganic & Medicinal Chemistry Letters 29 ( 2) 160 -163

1
2019
Synthesis and properties of 2'-O-methyl-2-thiouridine and oligoribonucleotides containing 2'-O-methyl-2-thiouridine.

Koh-ichiroh Shohda , Itaru Okamoto , Takeshi Wada , Kohji Seio
Bioorganic & Medicinal Chemistry Letters 10 ( 16) 1795 -1798

42
2000
Deoxynucleoside Triphosphate Containing Pyridazin-3-one Aglycon as a Thymidine Triphosphate Substitute for Primer Extension and Chain Elongation by Klenow Fragments.

Takahito Tomori , Kento Nagaoka , Leo Takeshita , Takashi Shiozawa
Journal of Organic Chemistry 83 ( 15) 8353 -8363

1
2018
Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine

Shi-Qi Cao , Itaru Okamoto , Hirosuke Tsunoda , Akihiro Ohkubo
Tetrahedron Letters 52 ( 3) 407 -410

11
2011