Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine
作者： Shi-Qi Cao , Itaru Okamoto , Hirosuke Tsunoda , Akihiro Ohkubo , Kohji Seio
DOI: 10.1016/J.TETLET.2010.11.065
关键词: Thermal denaturation 、 C nucleosides 、 Disulfide bond 、 Thermal stability 、 Stereochemistry 、 Thio- 、 Nucleoside 、 Chemistry 、 Organic chemistry 、 Oligonucleotide
摘要: Abstract We synthesized the 3′-phosphoramidite building block of 4-thiopseudoisocytidine (s 4 Ψ i C) and incorporated it into triplex-forming oligonucleotides (TFOs). The results thermal denaturation triplexes incorporating s C showed that could be used as a nucleoside component TFOs to increase stability at pH 7.
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