Triplex forming ability of oligonucleotides containing 2'-O-methyl-2-thiouridine or 2-thiothymidine.
作者： Itaru Okamoto , Kohji Seio , Mitsuo Sekine
DOI: 10.1016/J.BMCL.2006.02.019
关键词: Protein secondary structure 、 Base pair 、 Stacking 、 Chemistry 、 Molecule 、 Selectivity 、 Chemical synthesis 、 Hoogsteen base pair 、 Stereochemistry 、 Oligonucleotide
摘要: Abstract The triplex forming ability of oligonucleotides containing 2′- O -methyl-2-thiouridine (s 2 Um) and 2-thiothymidine T) was studied. UV melting experiments revealed that (TFOs) both s Um or T stabilized significantly parallel triplexes. main reason for stabilization triplexes due to the stacking effect 2-thiocarbonyl group. Moreover, it turned out these modified TFOs had a high selectivity in recognition matched Hoogsteen base from mismatched one.
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