Bisphosphonates induce apoptosis in human myeloma cell lines: a novel anti-tumour activity.

摘要: Bisphosphonates are in widespread use to prevent bone resorption a number of metabolic and tumour-induced diseases including multiple myeloma. Recent reports suggest that bisphosphonate treatment may be associated with an increase patient survival, raising the possibility these compounds have direct effect on tumour cells. We investigated whether bisphosphonates clodronate, pamidronate YM175 can directly affect human myeloma cell lines U266-B1, JJN-3 HS-Sultan vitro. The cycle progression was examined using flow cytometry. ability induce apoptosis determined basis changes nuclear morphology DNA fragmentation. Pamidronate more potent bisphosphonate, YM175, significantly decreased (P < 0.001) also caused cells arrest S-phase line. Both proportion altered (P < 0.05) fragmented DNA, characteristic apoptosis, both In contrast, clodronate had little did not cause at concentrations examined. These data raise some could anti-tumour effects vivo.