Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen.
作者： F. Salah , A. El Bassiouny , I. Rabia , Z. Demerdash , M. Roshdy
DOI: 10.1007/S00436-005-0016-8
关键词: Antigen 、 Echinococcus granulosus 、 Helminthiasis 、 Virology 、 Schistosomiasis 、 Schistosoma haematobium 、 Antibody 、 Fasciola hepatica 、 Monoclonal antibody 、 Biology
摘要: The present study was designed to prepare monoclonal antibodies (MAbs) against Schistosoma haematobium soluble egg antigen (SEA) with immunodiagnostic potential for urinary schistosomiasis. From a panel of MAbs, pair IgG1 MAbs (2D/11C and 10B/2C) specific S. SEA selected. Both recognized one band 42-kDa molecular weight by western blots. employed in sandwich ELISA the detection circulating schistosome (CSA), as antigen-capturing antibody other peroxidase-conjugated antigen-detecting antibody. lower limit assay 1 ng/ml SEA. performed on sera 65 haematobium-infected patients, 25 patients infected parasites (Fasciola hepatica, Echinococcus granulosus), 20 noninfected individuals. CSA demonstrated 89% group. However, negative healthy individuals parasites, giving an overall specificity 100% assay. A positive correlation (r=0.37, p<0.01) detected between number eggs excreted 10 ml urine level patients. Our data show that use anti-S. provides sensitive method immunodiagnosis active Moreover, using this MAb/ELISA system proven correlate intensity infection hence morbidity assessment.
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