Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study
作者： Faten S. Mahmoud , Zeinab A. Demerdash , Sherihan M. Elmotawakel , Salwa Hassan , Mohamed Hendawy
DOI: 10.1016/J.CEGH.2019.09.014
关键词:
摘要: Abstract Objective to assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and evaluate their diagnostic potential replace old conventional method animal ascetic fluid. Methods Highly reactive specific MAb secreting S. mansoni SEA was cultured three different media; rich replacement (conventional), (SLRM) free (SFRM). Results Cell count viability were better SLRM than SFRM. The lower detection limit assay Mab culture 1.1 ng/ml SEA. SFRM excluded because concentration reactivity. tested against 34 human samples. Sensitivity specificity for each 92% 90% respectively. efficacy 94%. Conclusions antischistosomal MAbs comparable on usage either vitro supernate or media.
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