Hsa_circ_0099198 facilitates the progression of retinoblastoma by regulating miR-1287/LRP6 axis.
作者： Fan Xiao , Lin Wang , Linlin Chen , Yanhua Jiang , Ting Wang
DOI: 10.1016/J.EXER.2021.108529
关键词: Cell cycle checkpoint 、 Carcinogenesis 、 Cell migration 、 Cell growth 、 Cell 、 Cancer research 、 Flow cytometry 、 Retinoblastoma 、 Real-time polymerase chain reaction 、 Chemistry
摘要: Abstract Retinoblastoma (RB) is an intraocular malignancy that occurs in children. Circular RNAs (circRNAs) have been confirmed to play essential role tumorigenesis and development. This study aimed ascertain the potential mechanism of hsa_circ_0099198 RB. The levels circ_0099198, microRNA-1287 (miR-1287) low-density lipoprotein receptor-related protein 6 (LRP6) were determined by real-time quantitative polymerase chain reaction Western blot. Cell proliferation was assessed colony formation assay. cycle arrest apoptosis evaluated flow cytometry. migration invasion tested using transwell activity caspase-3/caspase-9 examined with commercial kits. interaction among miR-1287 LRP6 verified dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay or pull-down Xenograft experiment used assess tumor growth vivo. circ_0099198 increased, while level reduced RB cells. silencing suppressed metastasis expedited cell Y79 So-RB50 In addition, depletion inhibited progression via regulating miR-1287/LRP6 axis. Moreover, knockdown blocked xenograft tumors. contributed sponging up-regulating LRP6, which provided novel biomarkers for therapy.
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