Fungal biosynthesis of endochitinase and chitobiase in solid state fermentation and their application for the production of N-acetyl-d-glucosamine from colloidal chitin
作者： P BINOD , C SANDHYA , P SUMA , G SZAKACS , A PANDEY
DOI: 10.1016/J.BIORTECH.2006.09.030
关键词: Biochemistry 、 Solid-state fermentation 、 Trichoderma harzianum 、 Glucosamine 、 Chitin 、 Chitinase 、 Endochitinase activity 、 Substrate (chemistry) 、 Fermentation 、 Chemistry
摘要: The present study was directed to the production of N-acetyl-D-glucosamine using endochitinase and chitobiase from fungal cultures in solid culturing. Fifteen strains were evaluated for under solid-state fermentation agro-industrial residues, which Penicillium aculeatum NRRL 2129 showed maximum activity whereas Trichoderma harzianum TUBF 927 activity. Eleven substrates, alone combination with chitin, enzyme production. Optimization physico-chemical parameters such as incubation period initial moisture content, nutritional chitin source, inorganic organic nitrogen sources, carried out. resulted more than 3-fold increase (from 3.5 12.53 U/g dry weight substrate) about 1.5-fold 1.6 2.25 substrate). Studies on degradation colloidal improved efficiency when used combination.
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