Fas mediates apoptosis and oxidant-induced cell death in cultured hRPE cells.

摘要: PURPOSE: To investigate whether Fas ligand (FasL) and the receptor system mediates apoptosis in cultured human retinal pigment epithelial (hRPE) cells contributes to oxidant-induced death of hRPE cells. METHODS: Expression FasL was examined by Western blot analysis flow cytometry. The susceptibility Fas-mediated determined incubating with recombinant soluble (sFasL). Characteristics assessed included chromatin condensation, DNA cleavage, phosphatidylserine exposure. possible involvement oxidative killing cells, effects oxidant tert-butylhydroperoxide (tBH) on expression were studied. specificity using antioxidants glutathione N-acetyl-L-cysteine (NAC). requirement for pathway tBH-induced investigated an antagonistic anti-Fas antibody ZB4 that blocks interaction between Fas. RESULTS: Cultured constitutively expressed Ligation sFasL triggered tBH treatment resulted increased Glutathione NAC completely abrogated increase apoptosis. Blocking inhibited apoptosis, but only partially. CONCLUSIONS: A functional apoptotic is present can be activated or upregulation oxidant. incomplete inhibition blocking indicates involved other triggering mechanisms are also important.