Biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species.

摘要: The formation of biofilms by diverse bacteria isolated from contaminated soil and groundwater on model substrata with different surface properties was assessed in a multifactorial screen. Diverse attachment phenotypes were observed as measured crystal violet dye retention confocal laser scanning microscopy (CLSM). Bulk measurements cell hydrophobicity had little predictive ability determining whether would develop hydrophobic or hydrophilic substrata. Therefore selected pairs the genera Rhodococcus, Pseudomonas Sphingomonas that exhibited examined more detail using CLSM lipophilic dye, Nile Red. association Rhodococcus sp. membranes lipids shown to influence these cells, but this approach not informative for Confocal Raman Microspectroscopy confirmed importance their indicated biofilms, nucleic acids proteins, respectively, important identifying differences strains. Treatment DNase I role predicted Pseudomonas. This work demonstrates microbes environmental samples varies markedly, range macromolecules may be involved differ significantly between genera. A combination spectroscopy distinguished could used identify key surfaces specific cases studied.