Raman spectroscopic differentiation of planktonic bacteria and biofilms.

摘要: Both biofilm formations as well planktonic cells of water bacteria such diverse species the Legionella genus Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were examined in detail by Raman microspectroscopy. Production various molecules involved formation tested nutrient-deficient media tap was observed particularly evident biofilms formed six species. Biofilms selected differ significantly from same organisms their lipid amount. Also, all have that other genera amount lipids they produced. We believe significant increase synthesis this molecular may be associated with ability to form biofilms. In addition, a combination microspectroscopy chemometric approaches can distinguish between both could used identify samples which unknown identification model. Our results provide valuable data for development fast reliable analytic methods based on microspectroscopy, applied analysis water-adapted microorganisms without any cultivation step.