Regulated viral BDNF delivery in combination with Schwann cells promotes axonal regeneration through capillary alginate hydrogels after spinal cord injury

摘要: Abstract Grafting of cell-seeded alginate capillary hydrogels into a spinal cord lesion site provides an axonal bridge while physically directing regenerating growth in linear pattern. However, without additional stimulus, bridging axons fail to extend the distal host cord. Here we examined whether combinatory strategy would support regeneration descending across cervical (C5) lateral hemisection rat Following transections, Schwann cell (SC)-seeded were grafted and AAV5 expressing brain-derived neurotrophic factor (BDNF) under control tetracycline-regulated promoter was injected caudally. In addition, SC injection caudal parenchyma further enhance re-enter Our data show that both serotonergic traced by biotinylated dextran amine (BDA) throughout scaffolds. The number is significantly increased when BDNF expression activated transient delivery able sustain after gene switched off. Descending are confined graft/host interface even with continuous for 8 weeks. Only SCs, pathway facilitating through host/graft generated allowing successfully Statement Significance Recovery from injury poor due limited observed adult mammalian central nervous system. Biomaterials, transplantation factors can guide site, provide cellular substrate, stimulate axon have shown some promise increasing distance axons. present study, combined biomaterial channels cells within beyond regulatable vector (BDNF). only full combination 4 weeks does not increase