Decrease in Cell Viability Due to the Accumulation of Spermidine in Spermidine Acetyltransferase-deficient Mutant of Escherichia coli
作者： Jun-ichi Fukuchi , Keiko Kashiwagi , Masahiro Yamagishi , Akira Ishihama , Kazuei Igarashi
关键词: DNA 、 Transformation (genetics) 、 Ribosome 、 Viability assay 、 Molecular biology 、 Escherichia coli 、 Mutant 、 Protein biosynthesis 、 Spermidine 、 Biology
摘要: Physiological functions of spermidine acetyltransferase in Escherichia coli have been studied using the (speG) gene-deficient mutant CAG2242 and cloned speG gene. The growth E. defined M9 medium was normal presence absence 0.5 mM spermidine. However, cell viability at 48 h after onset decreased greatly by addition amount accumulated cells approximately 3-fold that grown Transformation gene to recovered viability. Decrease observed even when added 24 growth. results indicate late stationary phase accumulation caused a decrease protein synthesis but not DNA RNA 28 several kinds proteins particularly inhibited. They included ribosome modulation factor OmpC protein. Since is essential for (Yamagishi, M., Matsushima, H., Wada, A., Sakagami, Fujita, N., Ishihama, A.(1993) EMBO J. 12, 625-630), thought be one reasons mainly occurred translational level.
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