Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein
作者： Chun-Xiao Wu , Wen-Pu Zhao , Hiroyuki Kishi , Junichi Dokan , Zhe-Xiong Jin
DOI: 10.1016/J.BBRC.2004.03.159
关键词: Nuclear protein 、 Electrophoretic mobility shift assay 、 Promoter 、 Zinc finger 、 Biology 、 Molecular biology 、 Regulation of gene expression 、 Transfection 、 Chromatin immunoprecipitation 、 Cell culture 、 Biophysics 、 Cell biology 、 Biochemistry
摘要: Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during lymphocyte development. Our previous study showed that -41 to -17 nucleotides (nt) 5' -upstream region of mouse were pre-requisite for core promoter activity Pax-5/c-Myb/LEF-1 protein-protein complex was responsible its immature B cells. In this study, we show -65/-42 sequence, non-conserved sequence between human promoter, is necessary full RAG-2. Electrophoresis mobility shift assay revealed Myc-associated zinc finger protein (MAZ) as well SP1/3 binds a GA box region. Using chromatin immunoprecipitation, MAZ pre-B Furthermore, synergistically activates murine with complex. These results first demonstrate participates activation promoter.
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